Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae

Authors

  • Fabio Demolinari de Miranda Universidade Federal do Espírito Santo, Centro de Ciências Agrárias, Departamento de Biologia
  • Andreia Barcelos Passos Lima Gontijo Universidade Federal do Espírito Santo, Centro Universitário Norte do Espírito, Departamento de Ciências Agrárias e Biológicas
  • Fabiano Costa Santiliano Universidade Federal do Espírito Santo, Centro de Ciências Agrárias, Departamento de Biologia
  • Fernanda Campanharo Favoreto Universidade Federal de Juiz de Fora, Instituto de Ciências Biológicas, Programa de Pós-graduação em Ecologia
  • Taís Cristina Bastos Soares Universidade Federal do Espírito Santo, Centro de Ciências Agrárias, Departamento de Farmácia e Nutrição

Keywords:

cross-amplification, SSR, genetic diversity, bromeliads, conservation

Abstract

Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult & Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.) Griseb., Aechmea ramosa, Billbergia horrida and Billbergia euphemiae, validating the transferability of these markers to species of the Pitcairnioideae and Bromelioideae subfamilies.
Issue Cover Vol.: 12 Num: 3

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Published

2012-09-01

How to Cite

Miranda, F. D. de, Gontijo, A. B. P. L., Santiliano, F. C., Favoreto, F. C., & Soares, T. C. B. (2012). Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae. Biota Neotropica, 12(3). Retrieved from //www.biotaneotropica.org.br/BN/article/view/1011

Issue

Vol. 12 No. 3 (2012): v12n3

Section

Short Communications

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