New isolates of Trichoderma antagonistic to Sclerotinia sclerotiorum

Authors

  • Eder Marques Universidade de Brasília, Instituto de Biologia, Departamento de Fitopatologia
  • Irene Martins Embrapa Recursos Genéticos e Biotecnologia
  • Mariana de Oliveira Cardoso Cunha Embrapa Recursos Genéticos e Biotecnologia
  • Marcello Arrais Lima Embrapa Recursos Genéticos e Biotecnologia
  • João Batista Tavares da Silva Embrapa Recursos Genéticos e Biotecnologia
  • Joseane Padilha da Silva Embrapa Recursos Genéticos e Biotecnologia
  • Peter Ward Inglis Embrapa Recursos Genéticos e Biotecnologia
  • Sueli Correa Marques Mello Embrapa Recursos Genéticos e Biotecnologia

Keywords:

Microbial genetic resources, molecular identification, biocontrol, white mold

Abstract

Abstract Forty-nine isolates of Trichoderma from the Brazilian Midwest were evaluated for their antagonistic activity in vitro against Sclerotinia sclerotiorum (causal agent of white mold), which were then identified based on their nuclear ribosomal ITS sequences. Paired culture tests showed that all isolates exhibited some antagonism, with a maximum of 77% mycelial inhibition and complete inhibition of sclerotia production. Two isolates were found to be the most promising biocontrol agents, considering both antagonistic parameters (CEN1253 - T. koningiopsis and CEN1265 - T. brevicompactum). Five different species were identified: T. harzianum (23), T. spirale (9), T. koningiopsis (8), T. brevicompactum (7) and T. asperellum (2). These isolates are stored in the Embrapa Fungi Collection for Biological Control and the information obtained in the experiments will be incorporated into the database of biological assets within the genetic resources information system (Allele) and be made available for further studies.
Issue Cover Vol.: 16 Num: 3

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Published

2016-01-01

How to Cite

Marques, E., Martins, I., Cunha, M. de O. C., Lima, M. A., Silva, J. B. T. da, Silva, J. P. da, Inglis, P. W., & Mello, S. C. M. (2016). New isolates of Trichoderma antagonistic to Sclerotinia sclerotiorum. Biota Neotropica, 16(3). Retrieved from //www.biotaneotropica.org.br/BN/article/view/1411

Issue

Vol. 16 No. 3 (2016): v16n3

Section

Articles

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